Miryam Cadenas established a new in vitro model for culturing HepG2 in spheroids during her ERASMUS stage in the lab of Bernhard Brüne (Frankfurt, Germany) (June 15-September 15 2017. Miryam showed that the necrotic nuclei of the spheroid is surrounded by an hypoxic area (green color in the image)
Mercedes Rubio-Manzanares Dorado presented her PhD last September 18, 2017. The study represents the first scientific collaboration between our group and the General Surgery Department of the Hospital University «Virgen del Rocío». The project entitled «Análisis de la viabilidad de los xenoinjertos de adenocarcinomas y tumores periampulares de pancreas» involved the implantation of adenocarcinoma from patients in nude mice. The jure of the thesis was constituted by Dr. Marçal Pastor Anglada (President), Dr. Miguel Ángel Gómez Bravo (Secretary), Dr. Miguel Ángel García Ureña (Vocal 1), Dr. Vicente Vega Ruiz (Vocal 2) and Dr. Carmelo Loinaz Segurola (Vocal 3).
I hope that the study will represent a starting point of scientific collaboration between the group of Surgery Department and Dr. Marçal Pastor who belong to the same programm in the CIBERehd.
Daniel Francisco Aparicio Sánchez presented his PhD last September 22, 2017. The study represents the first scientific collaboration between our group and the General Surgery Department of the Hospital University «Virgen del Rocío». The project entitled «Estudio histopatológico e inmunohistoquímico de xenoinjertos humanos de tumores periampulares» involved the implantation of adenocarcinoma from patients in nude mice. The jure of the thesis was constituted by Dr. Juan Sastre Belloch (President), Dr. Gerardo Blanco Fernández (Secretary), Dr. José María Álamo Martinéz (Vocal 1), Dr. Ignacio González Pinto Arrillaga (Vocal 2), Dra. Yiliam Fundora Sánchez (Vocal 3).
I have spent a very nice afternoon walking across all town with my friend Juan Sastre who I know from long time ago working in the field of free radical research!! Thank you Juan for coming to Seville!
María Negrete tracked experimental glycoprotein-based nanocompounds (CR1 and TM25) by fluorescence microscopy in HepG2 cells. The nanocompouds are able to encapsulate Sorafenib and other antitumoral drugs. The image (left panel) showed DAPI-stained nucleus from cultured HepG2 cells (6 hours). The image (center panel) showed the presence of TM25, and its localization around nucleus (right panel, merge DAPI and red fluoresce) in HepG2 cells (September 2017).
Published the first collaborative study with our colleague and friend Mario David Cordero Morales “NLRP3-inflammasome inhibition prevents high fat and high sugar diets-induced heart damage through autophagy induction. The study has been published in Oncotarget. 2017 Sep 8;8(59):99740-99756.
Thanks to the Short Term Scientific Mission (STSM) granted by the Mitochondrial mapping: Evolution-Age-Gender-Lifestyle-Environment (COST Action CA15203 MITOEAGLE) (20-30 November, 2017), I have initiated a new fruitful collaboration with the group of Professor Zuzana Červinková in the School of Medicine (Charles University, Hradec Kralove, Czech Republic). We have obtained a nice preliminary data regarding the regulation of mitochondrial respiration using Oroboros Oxygraph-2K in liver cancer cells. I am sure that a great future is open working together in this field. Thank you very much Zuzana, Pavla, Ondřej, Otto, René and Halka!. I hope to see you very soon!
The first scientific step with the Zuzana’s group has been already been accomplished!!!!!. Rodríguez-Hernández MA, de la Cruz-Ojeda P, Gallego P, Navarro-Villarán E, Staňková P, Del Campo JA, Kučera O, Elkalaf M, Maseko TE, Červinková Z, Muntané J. Dose-dependent Regulation of Mitochondrial Function and Cell Death Pathway by Sorafenib in Liver Cancer Cells. Biochem Pharmacol 2020 Mar 7, 113902. doi: 10.1016/j.bcp.2020.113902. PMID: 32156660.
María Negrete has succesfully obtained her TFM presenting the study entitled «Increase of the antitumoral activity of Sorafenib by new nanotechnological-based formulations» with the score of 9 out of 10 (November 11, 2017). The study has been done in collaboration with the group of Dr. Noureddine Khiar (Instituto de Investigaciones Químicas, IIQ, Isla de la Cartuja, Sevilla). Congratulations María Negrete!
Miryam Cadenas has successfully obtained her TFM presenting the study entitled «Histological markers related to nitrosative stress and cell death as prognostic markers of patients with hepatocarcinoma submitted to liver transplantation» with the score of 9.5 out of 10 (November 11, 2017). Congratulations Miryam!
Published the first collaborative study with our colleague and friend Jose Antonio Del Campo entitled “Simvastatin and metformin inhibit cell growth in hepatitis C virus infected cells via mTOR increasing PTEN and autophagy. The study has been published in PLoS One. 2018 Jan 31;13(1):e0191805.
María Ángeles Rodríguez and Raúl González have finally finished and get published the most relevant paper of the project started by Ángel José de la Rosa on the mechanism of induction of cell death by Sorafenib in liver cancer cells (ISCiii, PI13/00021; and CS, PI-00025-2013). The paper entitled “Molecular characterization of autophagic and apoptotic signaling induced by sorafenib in liver cancer cells” published in Journal of Cellular Physiology has just appeared in PubMed (PMID: 30132846). The paper has greatly involved the participation of Paloma Gallego, Raquel Ordoñez, Elena Navarro-Villarán, Laura Contreras, Mario Rodríguez-Arribas, Javier González-Gallego, José M. Álamo-Martínez, Luís M. Marín-Gómez, José A. Del Campo, José L. Quiles, José M. Fuentes, Jesús de la Cruz, José L. Mauriz, Francisco J. Padillo and Jordi Muntané. The recognition is for all of them. Thank you!!
Congratulations Patricia de la Cruz!!!.Mrs. Patricia de la Cruz Ojeda has been granted by the Ministry of Science, Innovation and Universities with the predoctoral fellowship FPU17/00026 (Call 2017) (48 months) to develop the doctoral thesis project entitled «Molecular characterization and regulation of the secretion of extracellular vesicles induced by the inhibitors of kinases and doxorubicin in liver cancer” at the Institute of Biomedicine in Seville (IBiS).
Published the first collaborative study with our colleague and friend Rocío Macias entitled «Serum metabolites as diagnostic biomarkers for cholangiocarcinoma, hepatocellular carcinoma and primary sclerosing cholangitis». The study has been published in Hepatology. 2018 Oct 16. doi: 10.1002/hep.30319.
María Orellana has succesfully obtained her TFM presenting the study entitled “Regulation of tyrosine kinase activity and its relation to the inhibition of cell proliferation and induction of apoptosis by Sorafenib in liver cancer cells” with the score of 8,5 out of 10 (December 10, 2018). The study has been co-headed by raúl González and Jordi Muntané. Congratulations María!
Friday, December 21, 2018. Christmas meal!!! Nice day guys!!!
Raquel Chapestro has succesfully obtained her TFM presenting the study entitled “Antitumoral properties of Sorafenib, Regorafenib, Cabozantinib and Lenvatinib in 3D cultured liver cancer cells” with the score of 9,03 out of 10 (April 9, 2019). The study has been co-headed by María Ángeles Rodríguez and Jordi Muntané. Congratulations Raquel!
Carmen Frías has succesfully obtained her TFM presenting the study entitled “Antitumoral activity and regulation of extracellular vesicles secretion by Sorafenib and Lenvatinib in hepatocellular carcinoma” with the score of 9,00 out of 10 (April 10, 2019). The study has been co-headed by Patricia de la Cruz and Jordi Muntané. Congratulations Carmen!
Excellent seminar «Mitochondrial cholesterol in liver disease» taught by Dr. José Carlos Fernández-Checa within XIX Ciclo Seminarios IBiS. High quality data and brainstorming for future projects (February 6, 2020). Thank you!!
The long lasting friendly and scientific relationship with de la Cruz’s group (Laboratory 201, IBiS) has reached the first scientific milestone!!! Congratulations! Thank you for your support Jesus!!!.
Navarro-Villarán E, de la Cruz-Ojeda P, Contreras L, González R, Negrete M, Rodríguez-Hernández MA, Marín-Gómez LM, Álamo-Martínez JM, Gómez-Bravo MA, de la Cruz J, Padillo J, Muntané J. Molecular pathways leading to induction of cell death and anti-proliferative properties by Tacrolimus and mTOR inhibitors in liver cancer cells. Cell Physiol Biochem. 2020 May 6;54(3):457-473. doi: 10.33594/000000230. PMID: 32369692
The major collaboration project carried out in the group has led to the publication of the study entitled “Downregulation of Thioredoxin-1-dependent CD95 S-nitrosation by Sorafenib reduces liver cancer” published in Redox Biol 2020;34:101528. doi: 10.1016/j.redox.2020.101528. PMID: 32388267.
Thank you to all collaborators from the University of Córdoba, Institute of Biomedicine of Seville, University of Valencia, Université Cote d’Azur, Bruker BioSpin Corporation and Hospital University «Santa Cristina».